It is amazing that such a small dipeptide molecule can have such a big role in the rejuvenation of the organism.
It is interesting that there was a rumor that Boris Yeltsin had taken for a long time ” Russian-super-vitamin ” –carnosine, and therefore looked 10 years younger.
Karnozin Extra has the remarkable ability to rejuvenate old cells and cells in the aging process and make them fully functional and healthy. Before there were known the results of research on carnosine, it was assumed that the old cells can not be rejuvenated. Recent studies have shown that carnosine increases the time between cell divisions, and at the same time increases the number of divisions. This means that the life of the cell is significantly extended, and the dependence is direct – more carnosine, the longer time period between the divisions and a greater number of divisions.
Numerous studies document that carnosine has great potential in the fight against aging because of its extraordinary ability to protect and extend the functional viability of key units that build the organism, such as cells, DNA, and lipids. Carnosine may rightly be called longevity substance. Given that this substance is absolutely safe, it is found naturally in the organism and food, and it was confirmed that prolongs the life of animals and cultures of human cell, it is considered a fundamental substance for prolonging life. Its antioxidant, protective, chelating and anti-glycation activities contribute to the normal cell functioning.
Characteristic of carnosine to slow down the aging process is not only a consequence of its antioxidant action. Another mechanism by which carnosine protects cells from oxidative stress is the ability to build the chelating agent, which were explained by the team of Professor Bruce N. Ames from Berkeley University in Los Angeles. Chelate formations with heavy metals – such as cadmium, copper, iron, mercury – prevent the participation of these metals in harmful reactions with peroxides.
The latest studies show that patients who take carnosine for a certain time (6 to 12 months), look younger than before use. This supports the findings of laboratory research and other experiments, which show that carnosine can rejuvenate cell culture as well as to suppress the visible signs of aging of living organisms (laboratory animals). During these examinations carnosine significantly delayed the development of skin wrinkles in the eye area, curvature of the spine, typical manifestations of aging. Moreover, not only the mice who received carnosine looked younger compared to those in the control group (non- carnosine), but they were also reaching 20% longer lifetime.
It is possible to explain anti age effect by comparing action of the pure carnosine and products of carnosine degradation in the blood stream, which is provided by specific carnosinase enzyme resulting in accumulation of histidine and β-alanine. In order to prove this differences, another living organisms were used in the following experiments where Drosophila melanogaster was used, which possess no carnosine metabolizing enzymes. In these experiments similar and even stronger life sustaining effect of carnosine was demonstrated were carnosinase was not present. This suggests that carnosine itself (not the products of its enzymatic degradation) demonstrates apparent life-sustaining action. In conclusion, use of a natural anti-oxidant and anti-glycating agent carnosine can open the new strategy in modern geroprotection.
Replication capabilities and number of cell divisions are reducing as cells are aging. Shortening of DNA telomere happens in each division (telomere = marginal DNA complex with a protein that protects the edges of chromosomes). This shortening of the DNA after each division, causes the formation of so-called signal of destruction of DNA (DNA-damage signal) and at a given moment is activated p53 (a tumor protein). Some types of chemotherapy induce premature aging like cancer, but also healthy cells. In contrast, carnosine has a strong ability to reverse the signs of aging of skin cells (fibroblasts) and to return their functionality, along with an extension of their lives. Such effects are typical effects of carnosine and are used for the prevention of all forms of harmful changes in the protein molecules and phospholipids, also for a significant limitation of DNA telomere shortening in order to prevent damage to DNA. Limit of the number of cell divisions is called Hayflick limit. This is the maximum number of cell divisions that occur before its destruction. Hayflick limit refers to cell mortality. In fact, most of the cells are regenerated when each by the division splits into two daughter cells . When cells reach Hayflick limit, there comes to the aging of the cell. Cells that age are still alive, but are no longer able to perform division, their structure and function are damaged.
Human fibroblasts are very suitable for cultivation and laboratory research. Elderly fibroblast culture cannot be replaced by younger ones, which are quite aligned and form groups of parallel fibers. In contrast, the old fibroblasts are granulated, of different sizes, not grouped and their fibers are irregular and of different sizes – they lose the ability to build the correct form. These important characteristics of the old cells are called adult phenotype (while the young cells represent juvenile phenotype). Under the guidance of a doctor McFarland, Australian scientist, are conducted a remarkable series of experiments which demonstrated that carnosine rejuvenates aging cells. Especially interesting is the following – when the aging cells are placed in culture enriched by carnosine, they show not only phenotypic changes from the old to the young, but increase their ability to division. They have once again proved their ability to organize, become aligned and build an organized group of fibers. When they return to a culture of non-carnosine, the signs of aging return quickly. When the same cells are again placed in culture with carnosine, they again show features of the juvenile phenotype. This was repeated with the same cells many times and always showed the same results. Moreover, carnosine significantly extended the life of old cells. Later, these experiments were confirmed by the British scientists led by Dr. Alan Hipkiss.
Carnosine delays the aging process in culture of human fibroblasts and can change the adult phenotype in juvenile phenotype. Regardless of the positive qualities of antioxidants to eliminate free radicals, they have never shown the ability to prevent aging as carnosine has. These are just combined, additional features of carnosine in action on the aging process. In particular, it is noted that carnosine is capable to react with carbonyl groups and to form a “carnozinilation” polypeptides (adducts), which suppresses the aging process and reduces the formations of damaged proteins, which are typical for these processes.
Revitalizing effect of carnosine on fibroblasts also explains why is significantly improved wound healing after surgical intervention.
The aging cells also produce adhesion molecules that cause thickening of the walls of blood vessels and their stiffness (atherosclerosis). The aging cells produce other additional degradative enzymes and anti-inflammatory cytokines, which act in distant parts of the body (they are transported through the blood). In this way, a relatively small amount of aged cells causes large changes in the function and integrity of the skin. The aging cells accumulate in all organs and tissues where they suffer apoptosis (programmed cell death) and summon the degenerative processes of aging. Moreover, the distortion of microscopic environment by accumulated aging cells can be reason for the increased incidence of malignant disease in older people.